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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Chitosan modified with PAP as a promising delivery system for melatonin in the treatment of osteoporosis: targeting the divalent metal transporter 1

Fig. 4

Effect of the slow-release system co-cultured in MC3T3-E1 cells, CS: chitosan (A). The 3-day CCK8 cell viability of MC3T3-E1 cells co-cultured with chitosan@PAP, MT, and chitosan@MT/PAP. (B). Immunostaining of MC3T3-E1 cells co-cultured with chitosan@PAP, PAP, and chitosan@MT/PAP. Boxed areas are shown at higher magnification on top. (C). Analysis of sepia areas. (D). Immunofluorescence staining of MC3T3-E1 cells co-cultured with chitosan@PAP, MT, and chitosan@MT/PAP. (E). Analysis of immunofluorescence staining. (F). RT-PCR of samples co-cultured with chitosan@PAP, MT, and chitosan@MT/PAP. n = 6 for each group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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Journal of Biological Engineering

ISSN: 1754-1611