Journal of Biological Engineering

Table 1 Collection of fluorescent tools provided in this study

From: Fluorescent tools for the standardized work in Gram-negative bacteria

FP^a	Maturation time (min)^b	Excitation^c	Emission^c	Quantum yield^a	Addgene reference
mCerulean [8]	6.6 [26]	452	479	0.46 [26]	213986
sfmTurquoise2ox [27]	N/A	455	478	0.93 [27]	213987
sfGFP [16]	13.6 [26]	492	512	0.64 [26]	213988
mGFPmut2 [7]	5.6 [26]	489	509	0.73 [26]	213989
eGFP [7]	25.0 [15]	494	511	0.67 [28]	213990
miniSOG [17]	N/A	453	522	0.47 [17]	213991
mVenusNB [29]	4.1 [26]	517	530	0.71 [26]	213992
Ypet [11]	N/A	517	530	0.76 [28]	213993
mNeonGreen [12]	10.9 [26]	506	519	0.80 [30]	213994
mRuby3 [30]	130.8 [26]	557	596	0.45 [30]	213995
mScarlet-I [14]	25.7 [26]	571	594	0.54 [14]	213996
mCherry [31]	15 [15]	585	611	0.22 [31]	213997
mCherry2-L [32]	22.8 [26]	583	613	0.22 [32]	213998
mNeptune2 [33]	591.5 [26]	600	651	0.24 [33]	213999
mNeptune2.5 [33]	34.5 [26]	597	639	0.28 [33]	214000
Tagged FPs	Tag^d		Addgene reference
mCerulean	LVA		214003
	AAV		214011
	ASV		214012
eGFP	LVA		214001
	AAV		214005
	ASV		214006
mNeonGreen	LVA		214002
	AAV		214010
	ASV		214008
mScarlet-I	LVA		214004
	AAV		214007
	ASV		214009

^aPrimary reference
^bMaturation times obtained from either FPbase (https://www.fpbase.org) or Balleza et al., 2018 as indicated. N/A: No information available
^cExcitation and emission optima experimentally obtained from spectra analyses (Figure S1)
^dSsrA tag amino acid sequence: LVA (RPAANDENYALVA), AAV (RPAANDENYAAAV), ASV (RPAANDENYAASV)

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ISSN: 1754-1611

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