Fig. 6
From: Fluorescent tools for the standardized work in Gram-negative bacteria
Visualizing cellular structures in bacteria using translational FP fusions. A Schematic representation of the bacterial flagellar motor highlighting the position of the FliG protein within the basal body structure. Figure adapted from Halte et al., 2021. [56]. B Histogram showing the signal-to-noise ratio of the FliG fluorescence foci of different fluorescent protein fusions (live cell), indicating the efficiency of each fluorophore for fluorescence focus observation. C Representative epifluorescence microscopy images displaying the distribution of the FliG protein fused to various fluorescent proteins within the bacterial cells (scale bar = 2 µm). D Representative confocal laser scanning microscopy (CLSM) images and super-resolution STED (Stimulated Emission Depletion) microscopy image of FliG-mNeonGreen fusion, showcasing the enhanced resolution of FliG protein localization using STED (scale bar = 1 µm)