Fig. 2

Evaluation of SsrA degradation tags that modulate the stability of FPs. A Relative fluorescence intensity of tagged FPs compared with their non-tagged counterparts. The tags cause a mild to moderate drop of maximum FP intensity that correlate with their affinity to be degraded. B + C Ratios of pulse-chase experiments (for the original data see Figure S3) to observe degradation speed over a time of 3 h after inhibition of de novo translation. Non-tagged FPs remain stable or show an increase in fluorescence intensity, due to maturation time of the FPs. The tagged FPs decrease in intensity at different speeds reflecting their degradation efficiency. For genome based tagged FPs, only fluorescence was detected for the ASV tag (see main text)