Fig. 1
From: Fluorescent tools for the standardized work in Gram-negative bacteria
Endpoint measurements of the fluorescent proteins evaluated in this study. A Relative fluorescent units normalized by optical density (OD600) are shown for cells constitutively (PrpsM) expressing the corresponding FP (colored bars), compared to the autofluorescence of S. enterica WT cells (white bars). The excitation and emission wavelengths for each FP are depicted in Table 1. Bar graphs represent mean values and standard deviations of at least three biological replicates. For evaluation of the spectra (Fig. S1) and endpoint measurements, cells were grown to exponential phase and fluorescence was directly measured in growth medium within a final volume of 200 µl in 96 well plates using a Synergy H1 plate reader. B Fold-change of reporter signal normalized to WT background fluorescence depicted for each FP