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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation

Fig. 2

SDS PAGE analysis of expression and purification of 4D5scFv-WT; WT and 4D5scFv-frTet variants (S122; 4D5scFv-S122frTet, G125; 4D5scFv-G125frTet, S127; 4D5scFv-S127frTet, P177; 4D5scFv-P177frTet, and G178; 4D5scFv-G178frTet). Proteins were separated using a 5% acrylamide stacking gel, followed by a 12% linear gradient gel. Lanes MW: Bio-Rad SDS PAGE dual color molecular weight standards ladder. Lanes BI: pre-induction samples. Lanes AI: post-induction samples. Lanes P: purified protein samples

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Journal of Biological Engineering

ISSN: 1754-1611