Fig. 6
From: The progressive trend of modeling and drug screening systems of breast cancer bone metastasis
Breast cancer-on-a-chip. a Ductal carcinoma in-situ (DCIS) formed in the lumen of the mammary duct, due to the accumulation of neoplastic epithelial cells, in the early stages of breast cancer; b Design of the DCIS microarchitecture; c Steps of creating DCIS microarchitecture: 1) separation of upper and lower cell culture chambers by collagen membrane. 2) Injection of collagen solution containing human fibroblast cells into the lower chamber 3) Continuous injection of HMF medium from the upper chamber and contraction of the gel due to the tensile force of fibroblasts 4) Create a Matrigel coating on the top surface. 5) Seeding of mammary epithelial cells. 6) Injection and adhesion of DCIS spheroids to epithelial cell, reprinted with permission from [168]