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Fig. 11 | Journal of Biological Engineering

Fig. 11

From: Challenges of aortic valve tissue culture – maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system

Fig. 11

Collagen quantification and tissue laminae after pulsatile dynamic vs. static AV tissue culture: A pAV tissues were stained with picrosirius red at the beginning of the experiment and after 14 days of high and low flow dynamic, static and lysis incubation conditions to reveal collagen fibres (left to right, representative samples shown, n = 3, scale bar: 500 µm); B MOVAT pentachrome stain was applied to assess trilaminar tissue stratification (C). A sponge-like ECM configuration appeared in lysis control (D); E Collagen fibres were quantified according to picrosirius red stain and relativized against cross section surface (two-way-ANOVA; * p < 0.05)

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Journal of Biological Engineering

ISSN: 1754-1611