Fig. 3
From: Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering
Transfection efficiency, expressed as luciferase activity normalized to the total protein content (RLU/mg of protein), following transfection with bPEI/pluc complexes prepared at N/P 10 on hMSCs isolated from two patients (i.e., patient #1 and patient #2, right and left panels, respectively) as a function of the pDNA dose (0.1, 0.5, and 1 µg/cm2) and the cell density (5 × 103, 1.5 × 104, 5 × 104, and 1 × 105 cells/cm2). Luciferase activity was evaluated 24, 48, and 72 h post-transfection. Results are expressed as mean ± SD (n = 3, * p < 0.05)