Fig. 4

Alterations of cell signaling pathways in the middle cerebral artery occlusion (MCAO) model. A Significant change in neural biological functions and diseases controlled by the dysregulated genes in the MCAO model. Color of Z-score represents the degree of activation or inhibition of the functions and diseases. B Activation of canonical pathways related to neural functions and inflammatory and immune responses. The color of the Z-score represents the degree of activation. C The gene network construction revealed the involvement of different ion channels, enzymes, receptors, transcription factors, and kinases in the activation of inflammatory and immune responses in the MCAO model. Red squares represent upregulated genes, green squares represent downregulated genes, orange arrows represent activated pathways, and blue arrows represent inhibited pathways. D Inhibition of canonical pathways related to neural functions. The color of the Z-score represents the degree of inhibition. E The Ingenuity Pathway Analysis demonstrated the contribution of different ion channels, enzymes, receptors, transcription factors, and kinases in the inhibition of neural functions in the MCAO model. Red squares represent upregulated genes, green squares represent downregulated genes, orange arrows represent activated pathways, and blue arrows represent inhibited pathways