Fig. 6

Interchain disulfide bonds introduced in different tetravalent T-cell engaging bispecific antibodies. (A) Expression of monoclonal antibodies (CEA-mAb and h2E-mAb) and BsAbs (CEA-BsAb-WT, CEA-BsAb, h2E-BsAb-WT and h2E-BsAb), was displayed by WB under non-reducing condition. Quantitative analysis for levels of monomer (B), expression (C) and purified yield (D). Mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001