Fig. 4

Cultivation data of E. coli BL21 (DE3) in 48-round-well high release ++ fed-batch MTPs. The OTR curves and the final pH-values at termination of E. coli BL21 (DE3) cultivations are presented. High release ++ fed-batch MTP in (c) and (d) were washed prior to cultivation for 24 h. a + c Varying culture volume per well (0.6 mL – 1.1 mL). b + d Varying inoculation OD_Start (0.1–2.0). Culture conditions (unless otherwise stated): pH₀ = 7.5, temperature T = 37 °C, humidity = 80%, shaking diameter d = 3 mm, shaking frequency n = 1000 rpm, culture volume V_L = 1000 μL/Well, OD_Start = 1.0, initial glucose concentration = 0 g/L. Shadow indicates standard deviation of six replicates. Symbols indicate every 10th data point