Fig. 10
From: Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer
Confocal microscopy of MG-63 cells seeded on ST1 and ST2 - day 14. Confocal microscopy of MG-63 cells seeded on ST1 (a, c) or ST2 (b, d) scaffolds from polylactic acid after a 14-day culture. Cells were fixed and cell membranes were stained using DiOC6 (3) (green), cell nuclei were stained with propidium iodide (red). Both maximum projections (a-b) and color coded projections (c, d), which display depth (d) distribution of cells (d = 180 μm in C, d = 200 μm in D) showed confluent layer of MG-63 cells and formation of bridges from cells connecting fibres on both scaffolds. Objective ×10, magnification ×2, Bar = 50 μm